Bioluminescent assay for total bile acids in serum with use of bacterial luciferase.
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چکیده
منابع مشابه
Bioluminescent assay for total bile acids in serum with use of bacterial luciferase.
This simple, rapid, sensitive kinetic bioluminescent method for the assay of bile acids in serum involves use of 3 alpha-hydroxysteroid dehydrogenase (EC 1.1.1.50) in combination with a new, commercially available NADH Monitoring Reagent (LKB-Wallac) containing a low activity of NADH:FMN-oxidoreductase and a high activity of bacterial luciferase. Interfering dehydrogenases in serum are inactiva...
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Total serum bile acid levels (TSBA) were determined in 44 patients with acute and chronic hepatic diseases. Elevated levels of TSBA were observed in all the patients. Values were higher in cirrhosis, lowest in liver cancer and intermediate in hepatitis. When compared with normal values a significant (p<0.001) increase in TSBA was found in three groups of patients. No significant correlation was...
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In this simple and direct method for determining total bile acids in serum, the serum was mixed with sodium pyruvate, a lactate dehydrogenase blocker, and bile acids were then measured spectrophotometrically after the following enzyme reaction. Bile acids are converted to 3-oxo bile acids with 3 alpha-hydroxysteroid dehydrogenase (EC 1.1.1.50) with concomitant reduction of NAD+ to NADH. The hyd...
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Sera of 119 apparently healthy subjects (63 males and 56 females) were analysed for total serum bile acids. The individuals were divided into five different age groups and findings between males and females were compared. A significant difference (p=0.02) was observed in serum bile acid levels between males and females in the age group of 25-34 years. The mean value was higher in females (Mean ...
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Proteases play important roles in a variety of disease processes. Understanding their biological functions underpins the efforts of drug discovery. We have developed a bioluminescent protease assay using a circularly permuted form of firefly luciferase, wherein the native enzyme termini were joined by a peptide containing a protease site of interest. Protease cleavage of these mutant luciferase...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1983
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/29.6.1123